| Range |
noradrenaline ~37,000: dopamine ~14,000 neurotransmitter molecules/vesicle
|
| Organism |
Rat Rattus norvegicus |
| Reference |
Courtney NA, Ford CP. The timing of dopamine- and noradrenaline-mediated transmission reflects underlying differences in the extent of spillover and pooling. J Neurosci. 2014 May 28 34(22):7645-56. doi: 10.1523/JNEUROSCI.0166-14.2014. p.7652 right column top paragraphPubMed ID24872568
|
| Primary Source |
Jaffe EH, Marty A, Schulte A, Chow RH (1998) Extrasynaptic vesicular transmitter release from the somata of substantia nigra neurons in rat midbrain slices. J Neurosci 18: 3548–3553. & Huang HP et al., (2007) Long latency of evoked quantal transmitter release from somata of locus coeruleus neurons in rat pontine slices. Proc Natl Acad Sci USA 104: 1401–1406.PubMed ID9570786, 17227848
|
| Method |
Amperometric measurements |
| Comments |
"The concentration of agonist determines the rate of activation of
GPCR [G-protein-coupled receptor]-mediated GIRK [G-protein inwardly rectifying potassium] currents (Sodickson and Bean, 1996 Ingram et al.,1997 Fordetal.,2009). Amperometric measurements have estimated somatic quantal content of noradrenaline vesicles in the LC [locus ceruleus] to be approximately twice that of dopamine vesicles in
the VTA [ventral tegmental area] (~37,000 noradrenaline molecules vs ~14,000 dopamine molecules per vesicle, primary sources). Despite the greater number of noradrenaline molecules being released per vesicle, the a2-dependent IPSC was nearly four times slower than D2-IPSC (Fig. 1C,F). This suggests that the a2-IPSC may be mediated by a subsaturating concentration of noradrenaline." |
| Entered by |
Uri M |
| ID |
111046 |