Average LuxR concentration at high cell density

Range ~575 Dimers/cell
Organism Bacteria Vibrio harveyi
Reference Teng SW. et al., Measurement of the copy number of the master quorum-sensing regulator of a bacterial cell. Biophys J. 2010 May 19 98(9):2024-31. doi: 10.1016/j.bpj.2010.01.031. p.2029 left column 3rd paragraphPubMed ID20441767
Method "[Researchers] report a sequence of experiments that combine the time-lapse and static snapshot approaches in V. harveyi to measure the copy number, N, of the master regulator protein LuxR, as well as its burst size, b, when LuxR is highly expressed. As in Rosenfeld and colleagues (refs 9,10), [researchers] determined the relative partitioning error of LuxR (fused to mCherry protein) at cell division by single-cell fluorescence time-lapse microscopy."
Comments "At low cell density, the average LuxR concentration, <Np>, is ~80 dimers/cell. At high cell density, ([AI-1]+[AI-2]=1000 nM), <Np> is observed to increase to ~575dimers/cell (Fig. 5 B), implying a sevenfold increase of LuxR concentration between the two limits." "V. harveyi communicates by synthesizing, releasing, and detecting the population-dependent accumulation of extracellular signal molecules called autoinducers (AIs)"
Entered by Uri M
ID 111011