Average number of luxR mRNAs transcribed during cell cycle

Range ~11 mRNAs/cell cycle
Organism Bacteria Vibrio harveyi
Reference Teng SW. et al., Measurement of the copy number of the master quorum-sensing regulator of a bacterial cell. Biophys J. 2010 May 19 98(9):2024-31. doi: 10.1016/j.bpj.2010.01.031. p.2025 right column top paragraphPubMed ID20441767
Method "[Researchers] report a sequence of experiments that combine the time-lapse and static snapshot approaches in V. harveyi to measure the copy number, N, of the master regulator protein LuxR, as well as its burst size, b, when LuxR is highly expressed. As in Rosenfeld and colleagues (refs 9,10), [researchers] determined the relative partitioning error of LuxR (fused to mCherry protein) at cell division by single-cell fluorescence time-lapse microscopy."
Comments "[Researchers] find that the burst size is ~50 dimers in the high-cell-density limit when LuxR is highly expressed. This implies that, on average, ~11 messenger RNAs are transcribed during a cell cycle. These are the first measurements of burst values of a key protein in a quorum-sensing network (b has been measured recently in E. coli using other techniques (refs 19,20))."
Entered by Uri M
ID 111008