Burst size in the high-cell-density limit when LuxR is highly expressed
| Range | ~50 Dimers/burst |
|---|---|
| Organism | Bacteria Vibrio harveyi |
| Reference | Teng SW. et al., Measurement of the copy number of the master quorum-sensing regulator of a bacterial cell. Biophys J. 2010 May 19 98(9):2024-31. doi: 10.1016/j.bpj.2010.01.031. p.2025 right column top paragraphPubMed ID20441767 |
| Method | "[Researchers] report a sequence of experiments that combine the time-lapse and static snapshot approaches in V. harveyi to measure the copy number, N, of the master regulator protein LuxR, as well as its burst size, b, when LuxR is highly expressed. As in Rosenfeld and colleagues (refs 9,10), [researchers] determined the relative partitioning error of LuxR (fused to mCherry protein) at cell division by single-cell fluorescence time-lapse microscopy." |
| Comments | "[Researchers] find that the burst size is ~50 dimers in the high-cell-density limit when LuxR is highly expressed...each mRNA produces ~50 LuxR dimers before it is degraded. This is a rather high translation rate. However, it is comparable with the large burst size (~100 monomers) measured in E. coli when the repressors completely dissociate from the Lac operon (ref 35)." |
| Entered by | Uri M |
| ID | 111007 |