| Range |
Table - link mmol/pH/Liter
|
| Organism |
Metazoa animals |
| Reference |
Roos A, Boron WF (1981) Intracellular pH. Physiol Rev 61: 296–434 p.399 table 13PubMed ID7012859
|
| Primary Source |
See pointers to refs on right of table |
| Method |
"Table 13 summarizes the available data on intracellular buffering power. The values obtained by titrating cell homogenates ideally reflect physicochemical buffering but may also reflect a degree of metabolic and organellar
buffering and some contamination from metabolic breakdown of the sample. In
some instances, extracellular contributions were not taken into account. The
remainder of the values were obtained by titrating intracellular fluid in situ,
either by exposing cells to weak acids and bases or by microinjection techniques.
The ?pHi measurements for these in situ data were all obtained with
microelectrodes. In some cases acid extrusion occurring after acute intracellular
acid loads may not have been totally compensated for. Experiments were
usually performed at an approximately normal pHo, so that passive fluxes of
acid or base are unlikely to have had a substantial effect." See methods on 3rd column from left |
| Comments |
"A considerable number of estimates of buffering power, many from in
vivo mammals, are omitted from Table 13 because they are likely to have been
heavily contaminated by active or passive transmembrane fluxes of acid or
base. The value of ß [buffering power] has long been estimated by changing PCO2 and then
measuring the resultant fall of pHi several hours later."
See notes beneath table |
| Entered by |
Uri M |
| ID |
110775 |