| Method |
"Cell preparations (77µg Chl ml^-1), containing the appropriate probe, were
scanned in the absence and presence of 95 mM of the broadening agent nickel
tetraphenylenepentaamine [NiTEPA (Q)]. Power was 10 mW, modulation
amplitude 1 gauss, time constant 0.064 sec, and the scan speed 10 cm/min.
Peak heights of the midfield lines were measured, and the values were corrected for reduction and normalized to a single gain setting (10, ref 4). Calculations were as described in the text. pHout was 7.10." |