Value |
23400
molecules/cell
|
Organism |
Budding yeast Saccharomyces cerevisiae |
Reference |
Carroll KM, Simpson DM, Eyers CE, Knight CG, Brownridge P, Dunn WB, Winder CL, Lanthaler K, Pir P, Malys N, Kell DB, Oliver SG, Gaskell SJ, Beynon RJ. Absolute quantification of the glycolytic pathway in yeast: deployment of a complete QconCAT approach. Mol Cell Proteomics. 2011 Dec10(12):M111.007633. doi: 10.1074/mcp.M111.007633. p.10 left column 2nd paragraphPubMed ID21931151
|
Method |
"[Researchers] developed the QconCAT approach for multiplexed
absolute quantification (refs 6, 7). In brief, synthetic genes,
optimized for heterologous expression in Escherichia coli,
encode a single open reading frame that is a concatenation of
tryptic peptides, each of which acts as an internal standard (a
Q-peptide) for a defined protein. Each analyte protein is represented
by at least one, but more preferably two (or more),
Q-peptides." |
Comments |
"Of the two
peptides selected for Pdc6p, IATTGEWDALTTDSEFQK and
LPVFDAPESLIK, the former was detected using an SRM [selected reaction monitoring] approach
with 23,400 molecules/cell obtained corresponding to
~800 amol on column for the most abundant extract." |
Entered by |
Uri M |
ID |
110557 |