| Range |
Table - link mutant frequency (uncertain units ?)
|
| Organism |
Mouse Mus musculus |
| Reference |
Busuttil RA, Garcia AM, Reddick RL, Dollé ME, Calder RB, Nelson JF, Vijg J. Intra-organ variation in age-related mutation accumulation in the mouse. PLoS One. 2007 Sep 12 2(9):e876. Supporting information Table S2. doi:10.1371/journal.pone.0000876.s002PubMed ID17849005
|
| Method |
"Using transgenic mice harboring a chromosomally integrated
lacZ reporter gene...To capture most of the epithelial cells, [researchers] scraped off the
innermost layer of the intestine (mucosa) with the surface villi.
Figure 5A shows the intact mucosa. [They] then determined the LacZ
mutant frequencies in the mucosal prep (Figure 5B) and in the
remaining fraction, which consists of the serosa, muscularis and
part of the lamina propia with some crypts still visible (Figure 5C).
For brevity [they] call this the serosa throughout the text. These two
fractions were analyzed for the duodenum, jejunum and ileum
from 3 to 6 mice per determination point at 19 months (Figure 6A,
Table S2)." |
| Entered by |
Uri M |
| ID |
110459 |