Activity of an average rrn operon in E. coli B/r at different growth rates

Range ~10 transcripts/min at 0.6 doublings/hour: ~60 transcripts/min at 2.5 doublings/hour transcripts/min
Organism Bacteria Escherichia coli
Reference Liang S, Bipatnath M, Xu Y, Chen S, Dennis P, Ehrenberg M, Bremer H. Activities of constitutive promoters in Escherichia coli. J Mol Biol. 1999 Sep 10 292(1):19-37. p.20 left column 2nd paragraphPubMed ID10493854
Primary Source Bremer, H. & Dennis, P. P. (1996). Modulation of chemical composition and other parameters of the cell by growth rate. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology, 2nd edit., pp. 97-1553-1569, American Society for Microbiology, Washington, DC.
Comments "...measurements of total RNA can be used to calculate the rate of rRNA synthesis in numbers of primary rRNA transcripts made per minute per given volume of culture. By combining such data with measurements of rrn gene dosage obtained from DNA replication data, the activity of an average rrn operon in E. coli B/r has been estimated to increase from about ten transcripts/minute at a growth rate of 0.6 doublings/hour to about 60 transcripts/minute at 2.5 doublings/hour at 37°C (primary source)."
Entered by Uri M
ID 110037