Concentration of ethyl methanesulfonate (EMS) used for mutagenesis

Range 32 mM
Organism Sugarcane Saccharum spp.
Reference Mahlanza T, Rutherford RS, Snyman SJ, Watt MP. In vitro generation of somaclonal variant plants of sugarcane for tolerance to Fusarium sacchari. Plant Cell Rep. 2013 Feb32(2):249-62. doi: 10.1007/s00299-012-1359-0. p.252 table 2PubMed ID23090725
Method "Embryogenic calli (0.2 g) were placed in 32 mM EMS solutions for 4 h, after which they were rinsed three times with liquid EMM (Embryo Maturation Medium) liquid EMM was used for the controls."
Entered by Uri M
ID 109869