Free energy for nucleotide addition in transcription
| Range | -3.5 kcal/mol |
|---|---|
| Organism | Generic |
| Reference | Encyclopedia of Biological Chemistry, e-only, S-H Jun, B A Warner, and K S Murakami, The Pennsylvania State University, University Park, PA, USA, RNA Polymerase Reaction in Bacteria, 2013 Elsevier Inc p.167 right column top paragraph |
| Comments | "The nucleotide addition is accomplished through the formation of a phosphodiester bond and release of pyrophosphate (PPi) as a by-product. The free energy for this reaction is rather small (?G=–3.5 kcal/mol), but additional free energy is provided in vivo by rapidly hydrolyzing PPi into two phosphate groups by enzyme pyrophosphatase that makes the entire reaction highly energetically favorable (?G=–7 kcal/mol BNID 109865) under physiological condition, which is practically irreversible." |
| Entered by | Uri M |
| ID | 109864 |