| Method |
"The nucleotide sequence was determined on DNA from purified phage particles of wildtype T7 (Studier, 1969,1979) by the methods of Maxam & Gilbert (Maxam, A. M. & Gilbert, W. (1977). Proc. Nat. Acad. Sci., U.S.A. 74, 560-564. Maxam, A. M. & Gilbert, W. (1979). In Methods in Enzymology (Grossman, L. & Moldave, K., eds), vol. 65, pp. 499-559, Academic Press, New York.), using previously determined restriction maps of T7 DNA as the starting point for isolating
specific DNA fragments (McDonell etal., 1977 Rosenberg etal., 1979 unpublished results). Computer programs for storing, searching and analyzing the nucleotide sequence
were developed and applied by K. Thompson and W. Crockett." |