| Range |
Table - link
|
| Organism |
Red stingray Dasyatis akajei |
| Reference |
Chong KT, Miyazaki G, Morimoto H, Oda Y, Park SY. Structures of the deoxy and CO forms of haemoglobin from Dasyatis akajei, a cartilaginous fish. Acta Crystallogr D Biol Crystallogr. 1999 Jul55(Pt 7):1291-300. p.1293 table 2PubMed ID10393295
|
| Method |
"Oxygen equilibrium curves were measured as described by
Imai et al. (1970). Measurements were carried out on purified
D. akajei Hb at 298 K in 50 mM 2-[bis(2-hydroxymethyl)
amino]-2-hydroxymethyl-1,3-propanediol (bis-Tris) or
Tris buffer with 100 mM chloride.In order to reduce
methaemoglobin levels, catalase and superoxide dismutase
were added to Hb samples (Lynch et al., 1976 Winterbourn et
al., 1976). Deoxygenation curves were used to determine p50
(the oxygen partial pressure necessary to attain 50% saturation
of Hb with oxygen) and the Hill coefficient n (the
maximum slope of Hill plots of oxygen equilibrium curves).
2 mM ATP was used as an organic phosphate effector." |
| Comments |
"The oxygen equilibrium characteristics are presented in
Table 2. D. akajei Hb shows a slightly lower oxygen affinity
than that of human HbA, with moderate haem-haem interaction,
Bohr effect and ATP effect (Imai, 1982)." See note beneath table |
| Entered by |
Uri M |
| ID |
109643 |