DNA replication speed in vivo
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|Organism||Bacteria Escherichia coli|
|Reference||Reyes-Lamothe R, Possoz C, Danilova O, Sherratt DJ. Independent positioning and action of Escherichia coli replisomes in live cells. Cell. 2008 Apr 4 133(1):90-102. doi: 10.1016/j.cell.2008.01.044. p.93 right column 2nd paragraphPubMed ID18394992|
|Method||"Fluorescent C-terminal fusion-protein derivatives of E. coli replisome components, expressed from their endogenous chromosomal promoters, were constructed and their phenotypes assessed by growth, flow cytometry, and microscopy."|
|Comments||"Five minutes of replication corresponds to ~180kb DNA replicated for each fork, ample time to give the observed spatial separation of two independent forks." From this one can infer a value of 600bp/s for the replication rate in vivo (180,000bp/300s).|
|Entered by||Uri M|