The relationship between the number of antigen-specific antibody secreting cells and the concentration of specific antibody from lymphocytes

Range Table - link
Organism Trout Oncorhynchus mykiss
Reference Bromage E, Stephens R, Hassoun L. The third dimension of ELISPOTs: quantifying antibody secretion from individual plasma cells. J Immunol Methods. 2009 Jul 31 346(1-2):75-9. doi: 10.1016/j.jim.2009.05.005. p.77 table 1PubMed ID19465022
Method "The enzyme-linked immunospot assay (ELISPOT), a technique widely used to enumerate the number of immune cells secreting a specific protein, such as antibodies or cytokines."
Comments "The fluorospots were enumerated from all wells containing between 5 and 100 spots. The number of spots was then multiplied by the dilution to give an estimate of how many antibody secreting cells there were for each tissue per 10^6 lymphocytes. Data for a single fish are shown in Table 1. The posterior kidney possessed an average of 5427 TNP-specific antibody secreting cells per 10^6 lymphocytes (c.a. 0.54%), while the blood contained only 67 TNP-specific antibody secreting cells per 10^6 lymphocytes (c.a. 0.0067%). Over a 12 h period, lymphocyte cultures from the spleen, anterior kidney, posterior kidney, and blood secreted 937, 1875, 3409,and 150 ng of TNP-specific antibody per 10^6 cells respectively (Table 1). On average peripheral blood antibody secreting cells secreted the most Ig (1.667 ng/cell), and splenic antibody secreting cells the least (0.399 ng/cell)." ASC=antibody secreting cells. TNP=trinitrophenol
Entered by Uri M
ID 108956