| Comments |
"The fluorospots were enumerated from all wells containing
between 5 and 100 spots. The number of spots was then
multiplied by the dilution to give an estimate of how many
antibody secreting cells there were for each tissue per 10^6
lymphocytes. Data for a single fish are shown in Table 1. The
posterior kidney possessed an average of 5427 TNP-specific
antibody secreting cells per 10^6 lymphocytes (c.a. 0.54%),
while the blood contained only 67 TNP-specific antibody
secreting cells per 10^6 lymphocytes (c.a. 0.0067%).
Over a 12 h period, lymphocyte cultures from the spleen,
anterior kidney, posterior kidney, and blood secreted 937,
1875, 3409,and 150 ng of TNP-specific antibody per 10^6 cells
respectively (Table 1). On average peripheral blood antibody
secreting cells secreted the most Ig (1.667 ng/cell), and
splenic antibody secreting cells the least (0.399 ng/cell)." ASC=antibody secreting cells. TNP=trinitrophenol |