| Range |
estimated ~1,000: predicted by Gaussian fitting 275 Ribosomes/cell
|
| Organism |
Bacteria Spiroplasma melliferum |
| Reference |
Ortiz JO, Förster F, Kürner J, Linaroudis AA, Baumeister W. Mapping 70S ribosomes in intact cells by cryoelectron tomography and pattern recognition. J Struct Biol. 2006 Nov156(2):334-41 DOI: 10.1016/j.jsb.2006.04.014 value of ~1,000 ribosomes per cell p.339 right column. Value of 275 ribosomes per cell p.338 right column and note beneath table 1. p.339 left column bottom paragraphPubMed ID16857386
|
| Method |
Cryoelectron tomography (CET) and Scaling index based segmentation (SIBS) |
| Comments |
Value of ~1,000 ribosomes per cell: P.339 right column: "Extrapolating the predicted ribosomal density to a whole cell, typically three to four times larger in volume than the analyzed portion, [researchers] can estimate that a Spiroplasma cell has a total complement of approximately 1000 ribosomes." Value of 275 ribosomes per cell: P.338 right column: "Considering that 275 ribosomes were predicted by Gaussian fitting of experimental cross-correlation peaks, [investigators] estimate that ca. 30% of all SIBS [Scaling index based segmentation] complexes are not ribosomes." P.338 note beneath table 1: "Gaussian fitting of the experimental distribution of CCCs [cross-correlation coefficients] predicts 275 ribosomes for the tomogram shown in Fig. 2." P.339 left column bottom paragraph: "Assuming that each 70S ribosome has a volume of 3400 nm^3 (Zhu et al., 1997), the predicted 275 ribosomes occupy approximately 5% of the cellular volume." |
| Entered by |
Uri M |
| ID |
108945 |