Range |
Table - link Protein/cell
|
Organism |
Human Homo sapiens |
Reference |
Zeiler M, Straube WL, Lundberg E, Uhlen M, Mann M. A Protein Epitope Signature Tag (PrEST) library allows SILAC-based absolute quantification and multiplexed determination of protein copy numbers in cell lines. Mol Cell Proteomics. 2012 Mar11(3):O111.009613. doi: 10.1074/mcp.O111.009613. p.9 table IIPubMed ID21964433
|
Method |
stable isotope labeling of amino acids in cell culture (SILAC) |
Comments |
"Given the
known amount of each PrEST (Protein Epitope Signature Tags) and their SILAC ratios with
respect to the endogenous proteins we determined the cellular
copy numbers of 40 different proteins. Very high accuracy
of absolute quantification to within a standard error of
25% was achieved for 35 of 40 proteins (Table II)." |
Entered by |
Uri M |
ID |
108912 |