| Range |
0.30 to 0.37 Table - link g/ml
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Zimmerman SB, Trach SO. Estimation of macromolecule concentrations and excluded volume effects for the cytoplasm of Escherichia coli. J Mol Biol. 1991 Dec 5 222(3):599-620. p.608 table 3PubMed ID1748995
|
| Method |
"Rough calculations based upon the composition of E. coli indicate a very high concentration of
macromolecules in the cytoplasm. A value of ˜0.62 g of cytoplasmic (RNA + protein) per g dry
weight of cells (based on total RNA and total protein values by Neidhardt (1987), allowing 8% of cell
dry weight as cell wall protein based upon pp. 246 and 255 of Salton (1964), and allowing 5% of cell
dry weight as periplasmic protein based upon the amount of protein released during preparation of
spheroplasts) taken with ˜1.5 to 1.8 g of cytoplasmic water per g dry weight of cells (based upon
Table 3 of Stock et al. (1977)) yields ˜0.34 to 0.42 g of (RNA + protein) per g of cytoplasmic water.
Assuming the specific volume [researchers] find for extract contents (0.67 ml/g), [researchers] expect concentrations of the order of ˜0.3 g of (RNA + protein) per ml of cytoplasm." |
| Comments |
"Two protocols are described in Materials and
Methods for measuring C[Cytoplasm], i.e. total (protein
+ RNA) concentrations in the cytoplasm, in terms
of sucrose-impermeable cell volume. The results of
these two methods are consistent with each other
and with the above estimate: C[Cytoplasm] values of
˜0.34 g/ml characterize both exponential and
stationary phase cells (Table 3). This value for
C[Cytoplasm] is converted with equation (10) to the
values for the excluded volume fraction, f, for
v=1.0 or 1.3 ml/g in Table 5." |
| Entered by |
Uri M |
| ID |
108879 |