Turnover of HSP60

Range ~0.08 Day^-1
Organism Thale cress Arabidopsis thaliana
Reference Li L, Nelson CJ, Solheim C, Whelan J, Millar AH. Determining degradation and synthesis rates of arabidopsis proteins using the kinetics of progressive 15N labeling of two-dimensional gel-separated protein spots. Mol Cell Proteomics. 2012 Jun11(6):M111.010025. doi: 10.1074/mcp.M111.010025. p.15 left column 2nd paragraphPubMed ID22215636
Method "[Researchers] used a (15)N labeling strategy with inorganic nitrogen sources coupled to a two-dimensional fluorescence difference gel electrophoresis and mass spectrometry analysis of two-dimensional IEF (Isoelectric focusing)/SDS-PAGE gel spots to define the rate of protein synthesis (K(S)) and degradation (K(D)) of Arabidopsis cell culture proteins."
Comments "...the slow turnover of HSP60 (At3g23990) involved in protein stabilization (0.08 d^-1)."
Entered by Uri M
ID 108860