| Range |
~0.08 Day^-1
|
| Organism |
Thale cress Arabidopsis thaliana |
| Reference |
Li L, Nelson CJ, Solheim C, Whelan J, Millar AH. Determining degradation and synthesis rates of arabidopsis proteins using the kinetics of progressive 15N labeling of two-dimensional gel-separated protein spots. Mol Cell Proteomics. 2012 Jun11(6):M111.010025. doi: 10.1074/mcp.M111.010025. p.15 left column 2nd paragraphPubMed ID22215636
|
| Method |
"[Researchers] used a (15)N labeling strategy with inorganic nitrogen sources coupled to a two-dimensional fluorescence difference gel electrophoresis and mass spectrometry analysis of two-dimensional IEF (Isoelectric focusing)/SDS-PAGE gel spots to define the rate of protein synthesis (K(S)) and degradation (K(D)) of Arabidopsis cell culture proteins." |
| Comments |
"...the slow turnover of
HSP60 (At3g23990) involved in protein stabilization (0.08
d^-1)." |
| Entered by |
Uri M |
| ID |
108860 |