| Range |
Table - link
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Bautista J, Satrústegui J, Machado A. Evidence suggesting that the NADPH/NADP ratio modulates the splitting of the isocitrate flux between the glyoxylic and tricarboxylic acid cycles, in Escherichia coli. FEBS Lett. 1979 Sep 15 105(2):333-6. p.335 table 4PubMed ID39785
|
| Method |
"Samples (100 or 200 ml) of E. coli cultures were
rapidly gathered in pre-cooled recipients and immediately
immersed in liquid nitrogen." |
| Comments |
"[Investigators] have measured the cellular concentrations of several metabolites whose variations in glucose and acetate-grown cells could give some insight into the actual occurrence of NADPH inhibition of E. coli IDH [NADP-isocitrate dehydrogenase]. The results are shown in table 4. The 5-fold increase in citrate and the 2-fold decrease in oxoglutarate found in acetate-grown cells is consistent with an actual inhibition of IDH. According to [investigators']
expectations, the NADPH/NADP ratio rises from
6.2-14 in glucose and acetate-grown cells, respectively,
thus supporting the regulatory role of this parameter
for the operation of the glyoxylate cycle." |
| Entered by |
Uri M |
| ID |
108042 |