Fraction of proteasomes present as 26 S complexes in HeLa cells
| Range | 50 to 60 % |
|---|---|
| Organism | Human Homo sapiens |
| Reference | Hendil KB, Hartmann-Petersen R, Tanaka K. 26 S proteasomes function as stable entities. J Mol Biol. 2002 Jan 25 315(4):627-36. p.628 right column top paragraphPubMed ID11812135 |
| Method | Quantitative immuno-blotting |
| Comments | Fractions with 26 S proteasomes and 20 S proteasomes, respectively, were pooled as indicated in Figure 1 and analysed by quantitative immuno-blotting for determination of core 20 S proteasomes. Results from such experiments showed that 50-60% of the proteasomes were present as 26 S complexes (data not shown), so as previously reported (PMID 10799514) roughly half of the proteasomes in HeLa cells are not associated with PA700. |
| Entered by | Uri M |
| ID | 108027 |