| Method |
"[Researchers] used fluorescence recovery after photobleaching
(FRAP) along with a detailed numerical model that simulates
FRAP experiments, to systematically investigate mobility
of cytoplasmic, inner membrane, and DNA-binding proteins
in Escherichia coli." "To characterize protein mobility in the E. coli cytoplasm and in the cytoplasmic membrane, FRAP experiments were performed for a series of 27–250 kDa cytoplasmic protein fusions to yellow and cyan fluorescent proteins (YFP and CFP, respectively), and of membrane protein fusions with 4–16 transmembrane helices ( Table 1 and Table S1)." |