| Comments |
"In contrast, however, the data obtained with the aminoacylated and nonacylated species generate different slopes and intercepts in 10 mM Mg2+ Aminoacylation results in a decrease
in Do20,w from (6.84±0.10)X10^-7 to (5.8±0.2)X10^-7
cm2/s and an increase in the slope from (0.10±0.02)
X10^-7 to (0.22±0.06)X10^-7(cm2/s)/(mg/mL). From the slope values, the average charge of nonacylated tRNAPhc is estimated to be 10±2e-, while the average charge of the aminoacylated form is 15±2e-, corresponding to an increase in negative charge of 5±2e-. Thus, while aminoacylation of tRNAPhe results in no changes in 1 mM Mg2+, an increase in negative charge and decrease in Do20,w are observed with the 10 mM Mg2+ condition." |