Ribosome footprint
| Range | ~28 Nucleotides |
|---|---|
| Organism | Unspecified |
| Reference | Ingolia NT. Genome-wide translational profiling by ribosome footprinting. Methods Enzymol. 2010 470:119-42. p.122 fig.6.1PubMed ID20946809 |
| Method | Ribosome footprints are generated by nuclease digestion of polysomes in cell extracts. Nuclease treatment degrades the mRNA that links together polysomes, freeing individual ribosomes (fig 6.1) |
| Comments | Ribosomes protect 28 nt mRNA footprint from nuclease digestion. |
| Entered by | Uri M |
| ID | 107874 |