RNA polymerase II footprint

Range ~40 Nucleotides
Organism Unspecified
Reference Selby CP, Drapkin R, Reinberg D, Sancar A. RNA polymerase II stalled at a thymine dimer: footprint and effect on excision repair. Nucleic Acids Res. 1997 Feb 15 25(4):787-93. abstract, p.787 right column 2nd paragraphPubMed ID9016630
Method "[Researchers] investigated the properties of the ternary complex of RNAP II at a T<>T [cyclobutane thymine dimer] in the template strand and the effect of the ternary complex on human excision nuclease."
Comments "The footprint of RNAP II stalled at the thymine dimer, obtained using DNase I, lambda exonuclease and T4 polymerase 3'>5'exonuclease, covers approximately 40 nt and is nearly symmetrical around the dimer." "[Researchers'] data show that RNAP II makes a stable ternary complex at the T<>T site (t1/2~20 h) in which RNAP II covers ~40 nt around the dimer in a nearly symmetrical manner."
Entered by Uri M
ID 107873