| Comments |
P.890 right column 2nd paragraph: "The photosynthetic machinery of LN [low nitrogen] beans was further characterized by quantifying the D1 protein of PS II, the large subunit of Rubisco and FNR [Ferredoxin-NADP+ reductase] in the leaves. N [nitrogen] deficiency did not affect D1 content, whereas the amounts of Rubisco and FNR were lower in LN than in HN [high nitrogen] plants, when measured on Chl [chlorophyll] basis (Fig. 2). Protein content per leaf area was only 10% lower in LN plants than in HN plants (Table 1), and therefore the low concentrations of FNR and Rubisco indicate specific down-regulation of these enzymes in LN plants. Only small differences in the Chl a/b and carotenoid/Chl ratio between LN and HN plants were detected, although the total Chl content per leaf area of LN plants was low (Table 1). PS II electron transport activities, measured from isolated thylakoids, were similar in HN and LN plants (Table 1). However, the maximal efficiency of PS II photochemistry (FM − F0)/FM, was lower in LN plants than in HN plants (Table 1). PS I activity, measured with MV as electron acceptor, was fairly similar in LN and HN beans (Table 1)." |