Intracellular buffering capacity as function of external pH

Range 0 to 470 figure - link nmol H+/pH unit/mg protein
Organism Bacteria Escherichia coli
Reference Krulwich TA, Agus R, Schneier M, Guffanti AA. Buffering capacity of bacilli that grow at different pH ranges. J Bacteriol. 1985 May162(2):768-72. p.771 fig.3PubMed ID3886633
Method "Acid-base titrations were conducted on whole cells and cells permeabilized with Triton X-100 [Bacilli spp.] or n-butanol [E. coli]." "Titrations of the untreated and permeabilized whole cells of the six bacterial species in the study immediately showed that both the Bo and the Bt values were quite different from species to species." Range extracted visually from fig.3
Entered by Uri M
ID 107129