| Range |
75 to 180 figure - link ng ion H+/pH unit/mg cell dry weight
|
| Organism |
Bacteria Staphylococcus aureus |
| Reference |
Collins SH, Hamilton WA. Magnitude of the protonmotive force in respiring Staphylococcus aureus and Escherichia coli. J Bacteriol. 1976 Jun126(3):1224-31. p.1227 fig.1PubMed ID7546
|
| Method |
Caption of fig.1:"Buffering powers of extracellular (Bo [black triangle]),
intracellular (Bi •), and total space (BT black square), of a
suspension of S. aureus. Cells were harvested in
exponential phase and allowed to equilibrate at a cell
density of 0.83 mg/ml in sucrose-choline chloride-potassium
chloride buffer as described in Materials
and Methods. Bo was determined from the instantaneous
change in pH on addition of anaerobic HCl. BT
was determined from the change in pH on addition of
HCl to cells treated with valinomycin and TCS (2µg/ml each). Bi is the difference between Bo and BT." Value extracted visually from fig.1 |
| Comments |
"The purpose of
the experiments reported in this communication was to obtain a measure of the magnitude
of the protonmotive force that is developed
across the cytoplasmic membrane of respiring
Staphylococcus aureus and Escherichia coli." "Figure 1 shows typical experimental
curves of the buffering power (dH+/
dpH) of a suspension of cells of S. aureus expressed
as nanogram-ions of H+ per pH unit per
milligram of cells (dry weight)." |
| Entered by |
Uri M |
| ID |
107126 |