| Range |
~27 Hours
|
| Organism |
Unspecified |
| Reference |
Baird GS, Zacharias DA, Tsien RY. Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral. Proc Natl Acad Sci U S A. 2000 Oct 24 97(22):11984-9. p.11986 left column top paragraphPubMed ID11050229
|
| Method |
"Quantum yields for photodestruction were
separately measured on a microscope stage or in a spectrofluorimeter." "For time trials of DsRed maturation, a
dilute solution of freshly synthesized and purified DsRed was
made in 10mMTris, pH 8.5, and this solution was stored at room
temperature in a stoppered cuvette (not airtight) and subjected
to periodic spectral analysis. For mutant maturation data, fluorescence
emission spectra (excitation at 475 nm or 558 nm)
were taken directly after synthesis and purification, and then
after .2 months storage at 4°C or room temperature." |
| Comments |
Red fluorescence reaches half its maximal fluorescence after
approximately 27 h and requires >48 h to reach >90% of
maximal fluorescence. |
| Entered by |
Uri M |
| ID |
107006 |