Longest sustained expression of a pore-forming protein in a liposome

Value 4 Days
Organism Unspecified
Reference Noireaux V, Libchaber A. A vesicle bioreactor as a step toward an artificial cell assembly. Proc Natl Acad Sci U S A. 2004 Dec 21 101(51):17669-74 p.17672 right column 1st paragraphPubMed ID15591347
Method To visualize the toxin and quantify the pore concentration as a function of time, a fusion a-hemolysineGFP was engineered with the reporter in the C terminus of the pore.
Comments expression is observed for >4 days with a maximum protein production between 30 and 40 µM
Entered by Uri M
ID 106925