Halflives of proteins in HEK293T human embryonic kidney cells

Range d1EGFP 1 hour: d4EGFP 4 hours: EGFP 24 hours Hours
Organism Mammalian tissue culture cell
Reference Yen HC, Xu Q, Chou DM, Zhao Z, Elledge SJ. Global protein stability profiling in mammalian cells. Science. 2008 Nov 7 322(5903):918-23. p.920 left column 2nd paragraphPubMed ID18988847
Method Researchers integrated the GPS reporter that expresses DsRed and EGFP under the control of the cytomegalovirus (CMV) promoter and the IRES from encephalomyocarditis virus into the genome of human embryonic kidney (HEK) 293T cells and analyzed cells by FACS (Fluorescence-Activated Cell Sorting).
Entered by Uri M
ID 106866