| Range |
2.33 and 29.7 mRNA molecules/hour
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Pelechano V, Chávez S, Pérez-Ortín JE. A complete set of nascent transcription rates for yeast genes. PLoS One. 2010 Nov 16 5(11):e15442. p.5 left column 2nd paragraphPubMed ID21103382
|
| Method |
Researchers developed genomic variants of
the well-known run-on technique to evaluate the nascent TR (transcription rate)
for most genes. In this technique (GRO, Genomic Run-on),
elongating RNA pol molecules, that conserve the RNA, are forced to incorporate radioactive UTP for a short length. |
| Comments |
If researchers assume that the 4670 genes
for which they have data are representative of the 5796 non-dubious
ORFs, the total transcription for RNA pol II in a yeast cell
growing in standard conditions is about 60200 mRNAs/h. |
| Entered by |
Uri M |
| ID |
106767 |