| Value |
0.12
mRNA molecules/min
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Pelechano V, Chávez S, Pérez-Ortín JE. A complete set of nascent transcription rates for yeast genes. PLoS One. 2010 Nov 16 5(11):e15442. p.5 left column 2nd paragraphPubMed ID21103382
|
| Method |
P.1 right column botom paragraph: "[Researchers] [ref 10] and others [refs 11,12] developed genomic variants of the well-known run-on technique [ref 13] to evaluate the nascent TR (transcription rate) for most genes. In this technique (GRO, Genomic Run-on), elongating RNA pol molecules, that conserve the RNA, are forced to incorporate radioactive UTP for a short length." |
| Comments |
p.5 left column 2nd paragraph: "The distribution of the TR values (Figure 3) is similar to a log-normal as shown in most cases by the expression datasets, being the median TR about 0.12 mRNA molecules/min (equivalent to 7 mRNAs/hour). Furthermore, 90% of the genes have TRs between 2.33 and 29.7 mRNAs/hour and, if [researchers] assume that the 4670 genes for which [they] have data are representative of the 5796 non-dubious ORFs, the total transcription for RNA pol II in a yeast cell growing in standard conditions is about 60200 mRNAs/h. By assuming the known datum that RNA polymerase molecules transcribe at 25 nt/s [ref 30], then the median RNA pol II density inside the genes is 0.078 molecules/kb." |
| Entered by |
Uri M |
| ID |
106766 |