In vitro residence time for glucocorticoid receptor (GR) in increased DNA conc. (22µg/ml)

Value 20 Sec
Organism Rat Rattus norvegicus
Reference Lieberman BA, Nordeen SK. DNA intersegment transfer, how steroid receptors search for a target site. J Biol Chem. 1997 Jan 10 272(2):1061-8. p.1064 right column 3rd paragraphPubMed ID8995403
Method A half-site oligonucleotide was used to measure the equilibrium binding constant K1 for GRdbd (glucocorticoid receptor DNA binding domain protein fragment) monomer binding to DNA.
Comments See BNID 106483, 106484, 106485. Nonspecific DNA increased the monomer dissociation rate to the limit of researchers' systems measurement (t1/2˜20s). Conc. of 22µg/ml is equivalent to 1×10^-6Mbp
Entered by Uri M
ID 106486