| Range |
34-44 Table - link %
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Zimmerman SB, Trach SO. Estimation of macromolecule concentrations and excluded volume effects for the cytoplasm of Escherichia coli. J Mol Biol. 1991 Dec 5 222(3):599-620. p.611 table 5PubMed ID1748995
|
| Method |
Using equation 10 p. 601: F=v×C(cytoplasm) where F is the volume fraction effectively occupied by cytoplasmic (protein+RNA), v the effective specific volume for
excluded volume interactions, and C(cytoplasm) conc. of RNA+protein in cytoplasm, and using C(cytoplasm)=0.34 g/ml and v=1.0 or 1.3 ml/g. |
| Comments |
In both stationary and exponential phase. This is about half the maximum packing
density of spheres in three dimension (Vazquez 2010 PMID 20171231). Note-range of 20-40% given by Ando et al., 2010 PMID 20937902 1st paragraph. Range of 10-15% given by Lukacs et al., 2000 PMID 10636854 p.1629 left column 3rd paragraph citing Kao et al., 1993 PMID 8416987 (however range wasn't located in Kao). |
| Entered by |
Uri M |
| ID |
105814 |