| Value |
20100
ribosomes/cell
Range: ±600 Table - link ribosomes/cell
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Nilsson M, Bülow L, Wahlund KG. Use of flow field-flow fractionation for the rapid quantitation of ribosome and ribosomal subunits in Escherichia coli at different protein production conditions. Biotechnol Bioeng. 1997 Jun 5 54(5):461-7. p.466 table IIIPubMed ID18634138
|
| Method |
Asymmetrical flow field-flow fractionation (asymmetrical flow FFF). For details see p.461 right column 1st paragraph link P.462 left column 5th paragraph: "As a control, the plasmid free strain E. coli TG1 was grown using identical conditions [in two 250-mL flasks containing 50 mL LB medium, see beginning of this paragraph in article] and a sample was withdrawn after 5 h and 25 min at OD550=3.3." |
| Comments |
P.466 left column top paragraph: "The results in Table III show that the number of ribosomes per cell decrease significantly in the presence of plasmids which can be explained by the burden on cell metabolism caused by the plasmids. The plausible effect is that more energy is directed toward maintaining the plasmid DNA than toward protein synthesis and thus less ribosomes are synthesized. Furthermore, [investigators] monitored the ribosomal composition of bacteria having induced plasmids (Fig. 5, fractogram 1) and used the bacteria with noninduced plasmids as control (Fig. 5, fractogram 2). The number of ribosomes per cell increased when the plasmid was induced (see Table III)." Number highly depends on growth conditions and stage. See BNID 101441, 105780 |
| Entered by |
Uri M |
| ID |
105779 |