| Range |
Table - link
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Gangola P, Rosen BP. Maintenance of intracellular calcium in Escherichia coli. J Biol Chem. 1987 Sep 15 262(26):12570-4. p. 12572 table 1PubMed ID2442165
|
| Method |
Free intracellular calcium was determined from fura-2 fluorescence and using the following equation: [Ca2+]i= Kd(F - Fmin)/(Fmax - F) where F is the fluorescence intensity of dye in the cells, Fmax and Fmin
are the intensities at saturating and zero calcium concentrations,
respectively, and Kd is the dissociation constant for fura-2 determined
under the appropriate conditions. |
| Comments |
The cytosolic concentration of free Ca2+
in the unstarved E . coli cells was determined to be 90±9nM
(n = 6). See column 2 in table for intracellular [Ca2+]. See BNID 100130. |
| Entered by |
Uri M |
| ID |
105669 |