| Range |
Table - link
|
| Organism |
Archaea Sulfolobus spp. |
| Reference |
Nunn CE, Johnsen U, Schonheit P, Fuhrer T, Sauer U, Hough DW, Danson MJ. Metabolism of pentose sugars in the hyperthermophilic archaea Sulfolobus solfataricus and Sulfolobus acidocaldarius. J Biol Chem. 2010 Aug 24, table 1PubMed ID20736170
|
| Method |
To confirm the activities and substrate
specificities of the enzymes in the proposed
pathway for C5 sugar catabolism (Fig. 2), and to
determine their kinetic parameters, the genes
encoding the enzymes have been cloned from S.
solfataricus and expressed in E. coli. In each
case, heat-treatment of the cell extract gave
recombinant enzyme that was of >90% purity,
although in some cases a chromatography step
was then carried out to achieve a homogeneous
preparation. The data are recorded in Table 1
and are compared with those found with enzyme
assays from cell extracts. |
| Comments |
In the current paper, researchers demonstrate that
Sulfolobus possesses a C5-specific dehydratase
that catalyses the dehydration of D-xylonate and
L-arabinonate to 2-keto-3-deoxy-D-xylonate
(KD-xylonate), and 2-keto-3-deoxy-L-arabinonate
(KD-arabinonate), respectively, and
the enzymes glycolaldehyde oxidoreductase and
glyoxylate reductase to catalyse the conversion
of glycolaldehyde to glycolate and then to
glyoxylate, which enters the citric acid cycle via
the action of malate synthase. See notes under table |
| Entered by |
Uri M |
| ID |
105663 |