| Value |
14
days
|
| Organism |
Tomato Lycopersicon esculentum |
| Reference |
Paungfoo-Lonhienne C, Rentsch D, Robatzek S, Webb RI, Sagulenko E, Näsholm T, Schmidt S, Lonhienne TG. Turning the table: plants consume microbes as a source of nutrients. PLoS One. 2010 Jul 305(7):e11915.PubMed ID20689833
|
| Method |
To examine if plants take up microbes and use them as a
nutrient source, researchers incubated roots of intact Arabidopsis and
tomato plants with E. coli Bl21 and yeast S. cerevisiae which express the green fluorescent protein ((GFP)E. coli and (GFP)yeast). Hydroponic tomato plants were incubated overnight
with GFPyeast. Since expression of GFP in yeast clone TDH3
(YGR192C) is constitutive, monitoring of GFP fluorescence allows
an assessment of yeast cells activity. |
| Comments |
Three hours after incubation,
fluorescing GFPyeast cells were detected at the root surface and
inside root cells (Figure 3A, p. e11916). After 3 days, GFPyeast was detected inside roots, with some yeast cells alive and fluorescing,
and some non-fluorescing yeast cells displaying an altered shape
(Figure 3A). Few yeast cells were fluorescing after 7 days, no GFP
signal was detected after 10 days, and root cells contained only
debris of yeast cells after 14 days (Figure 3A). |
| Entered by |
Uri M |
| ID |
105641 |