Number of cells in droplet

Value 1e+6 unitless
Organism Bacteria Escherichia coli
Reference Hallatschek O, Hersen P, Ramanathan S, Nelson DR. Genetic drift at expanding frontiers promotes gene segregation. Proc Natl Acad Sci U S A. 2007 Dec 11 104(50):19926-30. p. 19927 left column fig 1, note 'A' under figure and 8th line from top in textPubMed ID18056799
Method The two (immotile) strains of E. coli were grown overnight in separate liquid cultures at 37°C in rich growth medium (LB) supplemented with Ampicillin. After tenfold dilution in fresh medium, researchers mixed them to obtain cultures with different proportions of the two kinds of cells. Droplets of different sizes were then pipetted on LB-agar plates (1.5% Agar) with Ampicillin, and grown at 37°C for up to 84 h. Population growth and mutant distribution were monitored at regular time intervals by means of a stereo microscope.
Comments For number of cells in E. coli and yeast colonies see BNID 104458 and 100266, respectively.
Entered by Uri M
ID 105548