| Value |
8.14e+8
bp
|
| Organism |
Sea urchin Strongylocentrotus purpuratus |
| Reference |
Sea Urchin Genome Sequencing Consortium and researchers, The genome of the sea urchin Strongylocentrotus purpuratus. Science. 2006 Nov 10 314(5801):941-52. p. 942 middle column 2nd paragraph from bottomPubMed ID17095691
|
| Method |
"Sperm from a single male was used to prepare DNA for all libraries (tables S1 and S2) and whole-genome shotgun (WGS) sequencing. The overall approach was based on the "combined strategy" used for the rat genome (Gibbs et al, 2004 PMID 15057822), where WGS sequencing to six times coverage was combined with two times sequence coverage of BAC clones from a minimal tiling path (MTP) (fig. S1). The use of BACs provided a framework for localizing the assembly process, which aided in the assembly of repeated sequences and solved problems associated with the high heterozygosity of the sea urchin genome, without [researchers'] resorting to extremely high coverage sequencing." BAC=Bacterial Artificial Chromosome |
| Comments |
"Combining the BAC-derived sequence with the WGS sequence generated a high-quality draft with 4 to 5% redundancy that covered more than 90% of the genome while sequencing to a level of 8× base coverage (table S2). The assembly size of 814 Mb is in good agreement with the previous estimate of genome size, 800 Mb ± 5% (Hinegardner 1971 PMID 5544593)." See BNID 105518 |
| Entered by |
Uri M |
| ID |
105517 |