Effect of different nitrogen sources on the growth rate of several strains

Range Table - link
Organism Bacteria Vibrio parahaemolyticus
Reference Twedt RM, Novelli RM. Modified selective and differential isolation medium for Vibrio parahaemolyticus. Appl Microbiol. 1971 Oct22(4):593-9. p. 594 table 2PubMed ID4943272
Method Several strains were grown on mixtures of peptone, proteose peptone, and thiotone dissolved in 3% NaCl, 0.25%K2HPO4 in pH 7.2. Culture flasks were incubated at 35°C and 160 rev/min in a gyratory shaker (New Brunswick Scientific Co., New Brunswick, N.J.). Viable cell counts were prepared from serial dilutions, and turbidities of flasks were determined at appropriate intervals from 0 through 24 hr. The generation time for a given medium, determined from the maximum tangent of a semi-log plot of turbidity versus time, equaled the shortest time for doubling optical density. Duplicate determinations were averaged.
Comments Range in table is 14-24 min. Table 1 in article (p. 594) gives growth rates on conventional media in the range of 19-33min. Table 3 (p. 595) gives growth rates on bufferred peptone-salt media supplemented with additives providing a carbohydrate source or modifying carbohydrate metabolism, in the range of 14.75-19.5min.
Entered by Uri M
ID 105456