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Effect of different nitrogen sources on the growth rate of several strains

Range	Table - link
Organism	Bacteria Vibrio parahaemolyticus
Reference	Twedt RM, Novelli RM. Modified selective and differential isolation medium for Vibrio parahaemolyticus. Appl Microbiol. 1971 Oct22(4):593-9. p. 594 table 2PubMed ID4943272
Method	Several strains were grown on mixtures of peptone, proteose peptone, and thiotone dissolved in 3% NaCl, 0.25%K2HPO4 in pH 7.2. Culture flasks were incubated at 35°C and 160 rev/min in a gyratory shaker (New Brunswick Scientific Co., New Brunswick, N.J.). Viable cell counts were prepared from serial dilutions, and turbidities of flasks were determined at appropriate intervals from 0 through 24 hr. The generation time for a given medium, determined from the maximum tangent of a semi-log plot of turbidity versus time, equaled the shortest time for doubling optical density. Duplicate determinations were averaged.
Comments	Range in table is 14-24 min. Table 1 in article (p. 594) gives growth rates on conventional media in the range of 19-33min. Table 3 (p. 595) gives growth rates on bufferred peptone-salt media supplemented with additives providing a carbohydrate source or modifying carbohydrate metabolism, in the range of 14.75-19.5min.
Entered by	Uri M
ID	105456