| Value |
725
Fold
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Cho CM, Mulchandani A, Chen W. Altering the substrate specificity of organophosphorus hydrolase for enhanced hydrolysis of chlorpyrifos. Appl Environ Microbiol. 2004 Aug70(8):4681-5. Table - link PubMed ID15294802
|
| Method |
Directed evolution. Researchers used two rounds of DNA shuffling and screened about
12,000 mutants to improve the hydrolytic activity of an organophosphorus hydrolase (OPH) 725-fold
toward the toxin chlorpyrifos. |
| Comments |
The best variant (B3651) displayed a
725-fold increase in catalytic efficiency, and the measured
kcat/Km value of 2.2×10^8M^-1s^-1 is similar to the reported
value for hydrolysis of paraoxon by the wild-type OPH (9.9×10^7M^-1s^-1, Griffiths et al 2003, PMID 12505981), close to the diffusion-controlled limit of enzymatic reaction rate, see BNID 103 916, 105 087. |
| Entered by |
Uri M |
| ID |
105434 |