Diffusion coefficient of polyA mRNA in nucleoplasm of U2OS (human osteosarcoma) cells

Value 0.04 µm^2/sec
Organism Human Homo sapiens
Reference Molenaar C, Abdulle A, Gena A, Tanke HJ, Dirks RW. Poly(A)+ RNAs roam the cell nucleus and pass through speckle domains in transcriptionally active and inactive cells. J Cell Biol. 2004 Apr 26 165(2):191-202. Table - link PubMed ID15117966
Method Poly(A)+ RNA was visualized in nuclei of living U2OS (human osteosarcoma) cells by means of a tetramethylrhodamine (TAMRA)-labeled 2'OMe (U)22 probe that is complementary to the poly(A) tail of mRNAs. Kinetics measured by FLIP (Fluorescence Loss in Photobleaching) and FRAP (Fluorescence recovery after photobleaching)
Comments See BNID 105292
Entered by Uri M
ID 105294