Misincorporation rate in translation in luciferase

Range 1.5e-8 - 2.9e-6 Table - link mismatched/matched amino acid
Organism Bacteria Escherichia coli
Reference Ortego BC, Whittenton JJ, Li H, Tu SC, Willson RC. In vivo translational inaccuracy in Escherichia coli: missense reporting using extremely low activity mutants of Vibrio harveyi luciferase. Biochemistry. 2007 Dec 4 46(48):13864-73PubMed ID17985929
Method Measurements of specific activity of mutant luciferase in a histidine codon. Misincorporation of histidine inspite of mutation restores activity of enzyme. Ratio between active/inactive enzymes gives error rate.
Comments Note-See additional higher error rates in table (5.3e-7 - 4.1e-3), list of refs in table. See BNID 103 454, 105215
Entered by Uri M
ID 105223