| Value |
354
sec
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Elf J, Li G, Xie XS. 2007. Probing transcription factor dynamics at the single-molecule level in a living cell. Science 316:1191–94PubMed ID17525339
|
| Method |
fluorescence studies of single molecule-fusion protein of lac repressor and venus YFP. Kinetic analysis yields a time constant of ~59 s for the exponential rise in bound repressor after IPTG is diluted. Because there are about three repressors per cell, it would take at most {t} ~ 59s×2×3=354sec for a single lac repressor dimer in one cell to find a specific operator. |
| Comments |
For value of ~200 sec to find a free operator please see PMID 21423716 p.10 right column top paragraph |
| Entered by |
Uri M |
| ID |
105178 |