Half life of AraC protein in vivo

Value 60 min
Organism Bacteria Escherichia coli
Reference Kolodrubetz D, Schleif R. Identification of araC protein and two-dimensional gels, its in vivo instability and normal level. J Mol Biol. 1981 Jun 15 149(1):133-9.PubMed ID7031266
Method Label (pulse) chase experiment. In a pulse chase experiment, a chemical substance is labeled (usually radioactively) and after a certain time an excess amount of the same substance, unlabeled this time, is added. The activity of the substance in the system studied is determined by monitoring the level of label over time.
Comments The AraC protein is unstable in vivo and possesses a half-life of about 60 minutes.
Entered by Uri M
ID 105138